蛋白质一级结构分析

ProtParam User-provided sequence: 10 20 30 40 50 60 MLKKQEKFYY GGDYNPEQWD ESVWKEDMRL MKKAGVNYVS INIFSWARLQ PDEETYDFST 70 80 90 100 110 120 LDKIMDMLAE NGIGADLATA TAAPPAWLSR KYPDSLPVDK DGARFLPGSR QHYCPNSKDY 130 140 150 160 170 180 ARLAAKLVRK IAERYKNHPA LVMWHVNNEY GCHIAECYCD NCKKAFQTWL KEKYSTIENL 190 200 210 220 230 240 NKSWSTDFWS QRYYEWEEIC LPGKTPTFAN PMQQLDYKAF MDDSLLALYK MERDILKAYT 250 260 270 280 290 300 PDVPVMTNLM GLHKPVDGFH WAKEMDLVTW DAYPDPFDRI PYAQFMAHDL MRSLKKQPFL 310 320 330 340 350 360 LMEQAAGAVN WRAQNAVKTP GQMRLWSYEA AAHGADGIMF FQWRASQGGA EKFHSGMVPH 370 380 390 400 410 420 SGDEESRNFR EVVQLGNELK NLEKVTGSAY AADVAIVFDW KNWWALELDS KPSSLVTYIK 430 440 450 460 470 480 QLLPFYKVLH TRNIGVDFIH PDEAMDRYKV VFAPASYRVT KAFADKVKDY VEKGGYFVSN 490 500 510 520 530 540 FFSGIADEND RVYLGGYPGA YRDILGIYVE EFAPMKKGAV HQIRTGYGDA AIRVWEEKIH 550 560 570 580 590 600 LKGAEALAWF KDGYLAGSPA VTAHHCGKGK AYYIGTQPDE QYLSSLLKDI LQEADVRPAL 610 620 630 640 650 660 DAPRGVEVVV RKNGNEKYLF LLNHTDQVQF VNAGGTYLEL INGDTESETV RLSPRDVKIL QVIEK

References and documentation are available.

Number of amino acids: 665 Molecular weight: 76089.5

Theoretical pI: 6.06

Amino acid composition: Ala (A) 63 9.5% Arg (R) 29 4.4% Asn (N) 27 4.1% Asp (D) 47 7.1% Cys (C) 7 1.1% Gln (Q) 24 3.6% Glu (E) 42 6.3% Gly (G) 43 6.5% His (H) 17 2.6% Ile (I) 26 3.9% Leu (L) 54 8.1% Lys (K) 51 7.7% Met (M) 20 3.0% Phe (F) 28 4.2% Pro (P) 32 4.8% Ser (S) 31 4.7% Thr (T) 24 3.6% Trp (W) 19 2.9% Tyr (Y) 38 5.7% Val (V) 43 6.5% Pyl (O) 0 0.0% Sec (U) 0 0.0%

(B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 89Total number of positively charged residues (Arg + Lys): 80 Atomic composition:

Carbon C 3451 Hydrogen H 5221 Nitrogen N 907 Oxygen O 988 Sulfur S 27

Formula: C3451H5221N907O988S27Total number of atoms: 10594 Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 161495

Abs 0.1% (=1 g/l) 2.122, assuming all pairs of Cys residues form cystines

Ext. coefficient 161120

Abs 0.1% (=1 g/l) 2.118, assuming all Cys residues are reduced Estimated half-life:

The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 29.16 This classifies the protein as stable.

Aliphatic index: 75.14

Grand average of hydropathicity (GRAVY): -0.451

EXPASY ——protparam